Moh Su’i, Enny Sumaryati, Ricky Prasetyo, Dan Panca Eric
Background: Lauric acid is very useful as an anti-bacterial, Coconut oil contains lauric acid 48%. Objectives: This research aims to study activity of lauric acid as an anti-bacterial activity of pathogenic and spoilage. Lauric acid can be isolated from coconut endosperm by the method of hydrolysis using lipase endogeneus. Research using experimental methods. Media containing bacterial pathogens (Salmonella sp., E. Coli, and Stafilococus aureus) and spoilage bacteria (Micrococcus, Pseudomonas and Bacillus stearothermophilus) was added lauric acid with varying concentrations (0-75%). Then, determinated Minimum Inhibitory Concentration (MIC) and Minimum Kill Concentration (MKC). Results: The results showed that lauric acid can inhibit (MIC) Salmonella sp., E. Coli, and Stafilococus aureus at concentrations of 3.13%. As against Micrococcus at a concentration of 10%, Bacillus stearothermophilus at a concentration of 30% and Pseudomonas at concentrations of 50%. MKC lauric acid against Salmonella amounted to 3.13%, while E. coli and Staphilococus aureus 6.25%. MKC against Micrococus at a concentration of 30%, Bacillus stearothermophillus at concentrations of 50% and Pseudomonas at concentrations of 70%. Conclusion: Lauric acid was isolated from coconut endosperm can be killed bacterial pathogenic (Salmonella bacteria, Stafilococus aureus and E. Coli) with concentration lower than spoilage bacterial Micrococus, Bacillus stearothermophillus and Pseudomonas. © 2015 AENSI Publisher All rights reserved.
University of Widyagama Malang, Jl. Borobudur No. 25, Malang, 65128, Indonesia
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